Advantage

Molecular Hiving™

High Quality　Environmentally friendly　
High cost-efficiency

Molecular Hiving™

High Quality
Environmentally friendly
High cost-efficiency

High Quality

In-process test can be performed every step of the elongation process. The sample for the in-process test can be obtained easily by direct sampling from the reaction mixture. The test uses UHPLC and TLC and we can find impurities at 0.05% detection level. Resulted in the monitoring of the product in every step of the elongation steps, the best protocol of the process can be prepared and we can obtain high purity crude of the target peptide.

Yohei Okada, Rico Takasawa, Daisuke Kubo, Natsumi Iwanaga, Shuji Fujita, Kosuke Suzuki, Hideaki Suzuki, Hidehiro Kamiya and Kazuhiro Chiba*
Organic Process Research & Development 2019, 23, 11, 2576-2581.

Environmentally friendly

Molecular Hiving™ controls residual solvents according to ICH Q3C guideline and furthermore select CMR free organic solvents for the elongation process according to ” Regulation (EC) No 1223/2009 of the European Parliament and of the Council of 30 November 2009 on cosmetic products” . Molecular Hiving™ uses less quantities of raw materials and solvents than the traditional peptide synthesis.

Solvent for cosmetic products
Molecular Hiving™ Standard Solvents
Preferred	Usable	Undesirable	Banned
1-Butanol	2-propanol	1,2-Diethoxyethane	2-Methoxyethyl ether(Diglyme)
1-Propanol(pref.over2-prop)	Acetonitrile	Anisol	Benzene
2-Butanol	Cyclohexane	Cellosolves(Me- and Et-)	Carbon tetra Chloride
2-Methyl THF	Cyclohexanon	Chlorobenzene	Chloroform
Acetone	Di-isopropyl ether	Dichloromethane(close to banned)	Ethylenedichloride
Amylacetate	DMSO	Diethyl ether(close to banned)
Benzyl alcohol	Ethyl acetate	Dimethoxyethane(Glyme)
Cyclopentylmethylether(CPME)	Heptane	Dimethyl acetamide
Ethanol(pref.overMethanol),inc.,	Isooctane	Dimethyl formamide
Ethyl lactate	Methanol	Dioxane(close to banned)
Isoamyl alcohol	Methyl ethyl ketone	Ethyl benzene
Isobutyl acetate	Methyl isobutyl keone(MIBK)	Ethylene glycol
Isopropyl acetate	Methyl acetate	Hexane(s)(close to banned)
N-Buthylacetate	Methylcyclohexane	Methylformate(close to banned)
Water	t-Butanol	N-Ethyl Pyrrolidone(preferred over NMP)
	t-Butyl Ethyl ether	N-Methyl Pyrrolidone(NMP)
	t-Butyl Methyl ether	Pentane
	t-Butyl Methyl ether	THF
	t-Butyl Methyl ether	Toluene
	Xylene(s)	Trifluorotoluene(close to banned)

Solvent for cosmetic product	Molecular Hiving™ Standard Solvents	Preferred	1-Butanol	1-Propanol(pref.over2-prop)	2-Butanol	2-Methyl THF	Acetone	Amylacetate	Benzyl alcohol	Cyclopentylmethylether(CPME)	Ethanol(pref.overMethanol),inc.,	Ethyl lactate	Isoamyl alcohol	Isobutyl acetate	Isopropyl acetate	N-Buthylacetate	Water
Usable	Molecular Hiving™ Standard Solvents	2-propanol	Acetonitrile	Cyclohexane	Cyclohexanon	Di-isopropyl ether	CDMSO	Ethyl acetate	Heptane	Isooctane	Methanol	Methyl ethyl ketone	Methyl isobutyl keone(MIBK)	Methyl acetate	Methylcyclohexane	t-Butanol	t-Butyl Ethyl ether	t-Butyl Methyl ether	Xylene(s)
Undesirable	1,2-Diethoxyethane	Anisol	Chlorobenzene	Dichloromethane(close to banned)	Diethyl ether(close to banned)	Dimethoxyethane(Glyme)	Dimethyl acetamide	Dimethyl formamide	Dioxane(close to banned)	Ethyl benzene	Ethylene glycol	Hexane(s)(close to banned)	Methylformate(close to banned)	N-Ethyl Pyrrolidone(preferred over NMP)	N-Methyl Pyrrolidone(NMP)	Pentane	THF	Toluene	Trifluorotoluene(close to banned)
Banned	2-Methoxyethyl ether(Diglyme)	Benzene	Carbon tetra Chloride	Chloroform	Ethylenedichloride

Raw Materials	Molecular Hiving	Traditional Method
Organic Solvents	1/10　※1	1
Amino acid & Coupling Reagents	1/4　※2	1

※1Depends on AA number and sequence
※2Internal results

High cost-efficiency

Cost-efficient process of Molecular Hiving™ conducts the one-pot method of elongation using minimum quantities of raw materials, by high yield, high purity, and high performance of the operation.

•Raw material quantity is low.

•Reaction and work-up operation time is short.

•Special and new facility is not required.

•Batch size is high.